The increasing interaction between wildlife, domestic animals, and humans in urban environments poses significant public health risks due to zoonotic pathogen transmission. This study aims to identify the epidemiological patterns and risk factors associated with zoonotic diseases in densely populated cities. We conducted a cross-sectional study involving 1,500 participants across three major urban centers in South America, Asia, and the Middle East. Data were collected through extensive field surveys, laboratory analyses, and advanced statistical modeling. Our findings indicate a high prevalence of zoonotic diseases, primarily transmitted through close human-animal contact and inadequate sanitation measures. Notably, the emergence of novel pathogens was linked to environmental changes and increased urbanization. Effective disease surveillance and proactive public health strategies are imperative to mitigate these risks. The study concludes with recommendations for integrated surveillance systems and policy frameworks to enhance urban public health resilience against zoonotic threats.