The aim of this research paper is to find out the effect of organizational culture on knowledge management practices. A questionnaire is designed on the basis of construct adopted from existing literature. Data is collected from five organizations with response rate of 63% as only 256 respondents replied out of 410. Data analysis is done through SPSS and Amos. Results of this study shows that organizational culture has significant positive impact on knowledge management practices. Present study has also contributed both managerially and theoretically as it will help organizations to study the impact of organizational culture on knowledge management practices in an organization and also adds in existing literature. This study has also some future implications as improved knowledge management in an organization can results in better organizational performance and future studies can be conducted in this regard. Similarly mediating effect in this relationship can also be studied