Thirty two rats were divided into four groups (8 rats/each) named as 1, 2, 3 and 4. Group 1 were fed basal diet and served as a control group. Whereas, group 2 were fed the basal diet mixed with 1% cholesterol ( high cholesterol diet). Groups 3 and 4 were fed the high cholesterol diet mixed with fenugreek seed powder 5% and 10%, respectively. Total cholesterol, low density lipoprotein cholesterol (LDL-c) and alanine transaminase (ALT) activity were significantly increased (32.4 ± 0.2 mg/dl; 12.2 ± 1.5 mg/dl; 26.5 ± 0.6 IU/l) whereas, high density lipoprotein cholesterol (HDL-c) concentration was decreased significantly (11.9 ± 1.6 mg/dl) in rats fed high cholesterol diet compared to control group (28.1 ± 0.1; 6.0 ± 1.3; 22.7 ± 0.4; 13.9 ± 1.5), respectively. Inclusion of both concentrations of fenugreek in high cholesterol diet reduced serum total cholesterol (30.5 ± 0.1; 29.5± 0.5) and ALT activity (22.1 ± 0.5; 21.7 ± 0.7), respectively compared to rats fed high cholesterol diet and control groups. Histopathological findings revealed fatty changes in liver of rats fed high cholesterol diet which recovered by dietary supplementation of fenugreek seed powder. Only, inclusion of lower concentration of fenugreek in diet of rats fed high cholesterol reduced LDL-c concentration (6.2 ± 1.2) and increased HDL-c concentration (15.0 ± 1.0) compared to rats fed high cholesterol diet and the control group. Conclusively, inclusion of low dose of fenugreek (5%) in ration of rats was recommended than the high dose (10%).