The implementation of a “Virtual Instrument” developed in MatLab/Simulink that allows the analysis of the measurement of mechanical vibrations in rotating machines is presented. To accomplish this, we identified the main rotating machines used in industry, the parameters that can be relevant when an analysis of vibration is made, the typical vibration frequency spectra of certain electrical and mechanical failures, the most common regulations employed by the industry with respect to vibration levels in rotating machinery, the tools that are used for vibration analysis, and tools for developing MatLab software that includes features for storing and managing data from a data base, also allowing an analysis and diagnosis of vibration in rotating machines.\nThe development cost for this virtual instrument is very low compared with the tens of thousands of dollars of their equivalents now available in the market.