The investigation illustrates the effects of different concentrations of Ni2+ and its combined applications with chelators (EDTA, EDDHA and DTPA), organic acid (Citric Acid) and metal ions (Zn2+ and Mg2+) on various biochemical attributes and its bioaccumulation in two crop plants (Triticum aestivum L. cv. UP 262 and Brassica campestris L. var. Toria) grown in amended soils during plant growth and development in pot culture experiments. There was significant enhancement in root length with increasing NiCl2 concentrations but it consistently reduced when treated with concentrations beyond 24 mg and 60 mg of NiCl2/kg dry soil in wheat and mustard seedlings respectively. The wheat seedlings treated with NiCl2-Mg2+/ NiCl2-Zn2+ showed a boost in the fresh weight unlike addition of chelating agents like CA / EDTA. The treatments with 120 mg of NiCl2/kg dry soil in combination with MgSO4 exhibited high root and shoot fresh weight in mustard seedlings. The dry biomass of roots and shoots of mustard seedlings was enhanced with Ni–MgSO4 (120 mg of NiCl2/kg dry soil) treatment. The Seedling Vigour Index (SVI) was found higher in 23 days old wheat and mustard seedlings when treated in 4 mg and 24 mg of NiCl2/kg dry soil respectively. Treatment with NiCl2-ZnSO4 (120 mg of NiCl2/kg dry soil) exhibited maximum Tolerance Index in 23 days old wheat and mustard seedlings. When treated with NiCl2-Zn2+ (120 mg of NiCl2-Zn2+ /kg dry soil), there was enhanced total chlorophyll content in wheat seedlings. Similarly, NiCl2-EDTA treated mustard seedlings showed high values of total chlorophyll content. The Fo value was high in wheat seedlings by the application of DTPA (120 mg of NiCl2/kg dry soil). The Fm value was high in 23 days old wheat and mustard seedlings indicating high variation of OJIP transient by the applications of NiCl2-ZnSO4 (120 mg of NiCl2/kg dry soil). The application of NiCl2-DTPA (120 mg of NiCl2/kg dry soil) significantly increased the values of protein content in wheat and mustard seedlings. The use of NiCl2-CA / NiCl2-DTPA (120 mg of NiCl2/kg dry soil) treatment showed drastic increase in proline content in 23 days old wheat and mustard seedlings respectively. Application of 120 mg of DTPA-NiCl2/kg dry soil treatments showed elevated transportation index in wheat and mustard seedlings. Total Accumulation Rate was more with NiCl2-CA / NiCl2-EDDHA (120 mg of NiCl2/kg dry soil) treatment in wheat and mustard seedlings respectively.