Medicinal plants play an important role for health care.Medicinal plants have ability to cure \nboth infectious and non-infectious diseases1\n.The present investigation was aimed to examine \nthe antimicrobial potential of DMSO crude extracts of Aloe barbadensis Miller ( Aloe vera) gel \nagainst the selected pathogens Bacillus subtilis, Salmonella typhi, Escherichia coli, \nStaphylococcus aureus, Proteus vulgaris, Aspergillusfumigatus, Candida albicans and \nPenicilliumsps. The bacteria were identified and confirmed by conventional microbiology \nprocedure.Antimicrobial study was carried out by disc diffusion method against the pathogens by \nusing the crude DMSO extracts of A.Vera gel. The antibacterial activity has been observed in \nthe DMSO gel extracts of A. Vera against all the tested bacteria with varied activity. The \nmaximum zone of inhibition 14 mm for E. coli, 11 mm for P. vulgaris, 12 mm for S. aureus and \n10 mm for B. subtilis were observed. The maximum zone of inhibition 10.5 mm for C. \nalbiciansand 8 mm for Penicilliumsps were observed.It is hoped that this study would lead to the \nestablishment of some compounds that could be used to formulate new and more potent \nantimicrobial drugs of natural origin.