Nanoporation and bio-electric effect on cancer cells exposed to an specific pulse is gaining applications in medical treatments, especially for cancer treatment. The effectiveness of the cancer treatment depends on specification of electric pulse and the conductivities and relative permittivities of the cell . But till now the information regarding the pore formation and ion uptake on intraorganelle for cancer cell are limited. In this research, the intraorganelle nanopore formation and ion uptake of osteoblast and tumor cells ,under the the influences of specific programmable of pico pulse generator and specific micro chip, were studied .By using the MATLAB and COMSOL , to elucidate the difference in responses of the normal and tumor cells for a given input pico pulse. A comparison of the induced intra organelle potential,current,pressure,surface tension,pore density and ion uptake of the tumor cells against normal cells shows that nearly 40% increase of nanoporation was observed in thetumor cell compared to the normal cell for a dedicated pico pulse in 3D advanced micro chip. And it is control by external user. But the threshold pulse duration is 〖10〗^(-12) and 〖10〗^(-15) for normal cell and cancer cell respectively.