Because the gliadins tightly linked to the low molecular weight glutenin subunits, they have indirectly important role in bread-making quality in terms of dough strength. This experiment was conducted in order to comparison of gliadin band patterns of Aegilops cylindrica ecotypes with wheat cv. Bezostaya. The results showed that five block types of gliadin coding-genes existed in Ae. cylindrica. The first block had alleles similar to Gli-A1g locus corresponding to wheat gliadin locus. The second block consisted of alleles similar to Gli-B1h locus and could be probably originated from a mutation in this gliadin coding-gene influencing the electrophoretic mobility. The third block included alleles such as Gli-A1o/A1d, which may be originated through intralocus recombination. The fourth block consisted of alleles Gli-D1i, which could be originated through the loss of the fastest or lowest component of the block. The last block included Null-allele. Our results confirmed that the gliadin proteins were not a useful marker in discriminant of different ecotypes of this species.