Bishop’s weed (Trachyspermum ammi L.) is an important medicinal, aromatic and spice plant of South, West and Central Asia. The study reports multiple shoot regeneration from seeds (embryo with two cotyledon explants) of bishop’s weed used as explant and cultured on 1.0 × MS medium containing 0.1-1.6 mg l-1 Kin or TDZ with and without 0.10 mg l-1IBA (10 combinations); and 0.5 plus 2.50 mgl-1 BA with and without 0.25 mg l-1 IBA (10 combinations); supplemented with 3% (w/v) sucrose and 0.65% (w/v) agar. Shoot regeneration ranged 1.73 to 2.14 shoots per explant on 1.0 × MS medium containing variants of Kin with and without IBA. Whereas, multiple shoot induction was recorded on 1.0 × MS medium containing variants of TDZ and variants of BAP with and without IBA that ranged 4.05 to 9.73 shoots per explant and 5.74 to 10.05 shoots per explant respectively. The results confirmed that 1.0×MS medium containing variants of Kin with and without IBA were least suitable for micropropagation purposes. Whereas, 1.0 × MS medium containing variants of BAP with and without IBA induced maximum number of shoots per explant compared to other regeneration media irrespective of the concentration of plant growth regulators. The results further testified successful induction of roots on 1.0 × MS medium containing 0.80 mg l-1 IBA supplemented with 75 g l-1 sucrose in growth chamber where the plants set seeds under 16 h day length conditions and ambient conditions of temperature and humidity after 8 weeks.