Neonatal calf diarrhea is supposed to be the main cause of production losses in dairy industry in Pakistan. This usually occurs due to improper management of calf after parturition or in early hours of life. To bring down these losses trails were conducted on 30 neonates. These were divided into 6 equal groups naming A, B, C, D, E and F by keeping “F” group as control. Excluding control group rest of the groups were treated with Colimune Ora, Cosumix Plus, Streptomegma, NMK Powder and Biovet respectively to as prophylactic measure. To check serological values the blood samples were collected at different intervals i.e. just after birth, 36 hours and 28 days. Overall increasing pattern in packed cell volume (PCV) and decreasing pattern in differential leukocyte count (DLC) was recorded but the results for total erythrocyte count (TEC) were variable. An increase of 16.57%, 93.47% and 96% in case of group A, B and D but a decrease of 8.72% and 5.06% in case of C and E were noted respectively after 36 hours. The overall results of experiment reveled no unwanted change in hematology or serum profile referring the drugs can be used for prophylactic measures.