Acyl carrier protein (ACP) is a small acidic protein that acts as an essential cofactor in many biosynthetic pathways depending on acyl transfer reactions. In this study, a new full-length cDNA of JcACP was obtained by RT-PCR technique from the energy plant Jatropha. The nucleotide sequence analysis of the JcACP gene revealed that Jatropha JcACP gene encodes a protein of 137 amino acids that belongs to PP-binding superfamily. Sequence alignment showed that its deduced amino acid sequence had high similarity with other ACP. qPCR analysis showed that JcACP was expressed in different tissues including the seed, leaf, root, flower, stem and pericarp. The JcACP gene displayed a bell-shaped pattern of expression, which had peak expression at 50 DAF. The results demonstrated that JcACP genes are required for lipid biosynthesis involved in both endosperm development and storage lipid accumulation.