This study was carried out at Central Anatolian Province in 2003 and 2004. Sward\nformed 7 m x 12 m. Nitrogen was applied six doses (annual rate of fertilizer N) as organic\nnitrogen fertilizer (0, 10, 20, 30, 40 and 50 kg N ha-1). Each parcel was harvested 3 times\n(First year, 20 mai, 18 July and 15 September; second year, 16 mai, 20 July and 14\nSeptember). Grass samples from each plot were determinated botanical composition and than\nchemically were analysed. Festuca ovina, Tymus, Astragalus sp., Artemisia and Agropyron\nrepens were dominant species. Increasing of N rate from 0 to 50 kg N ha-1, botanical\ncomposition has rised constantly until 40 kg N ha-1. In 50 kg ha-1, plant number decreased.\nAccording to results of this study, harvest time effects significantly on chemical\ncompositions.