Commiphora gileadensis is a plant tree in the Burseraceae family that was cultivated in ancient times. The plant is also known as balsam, was renowned for the expensive perfume that was produced from it, as well as for exceptional medicinal properties that were attributed to its sap, wood, bark, and seeds. The aim of this study was to evaluate the variants for assessing genetic relationships and diversity in Commiphora Gileadensis species collections. Selected plant species were collected from Al-Baha and Al-Jizan region from Kingdom of Saudi Arabia. DNA was extracted from the selected plant species using the phenol-chloroform method and polymerase chain reaction (PCR) was performed with the specific primers chosen from ITS2 gene. Later on, DNA sequencing was performed to find out the presence of mutation. In this study, we did not find any mutation after performing the PCR and DNA sequencing. In conclusion, we cannot find any mutation from the plant species B and J from Saudi Arabia.