In this study overwintering sites of ladybeetls (Col.: Coccinellidae) was studied during 2010 in Mehriz district (Yazd province, Iran). Adult specimens were collected by hand using screwdriver underneath the bark of old trees. Adults of some coccinellid species overwinter in aggregations consisting of many individuals. Many of the ladybirds overwinter in a group, as they may be attracted to a particular environmental stimulus or of an innate tendency to join conspecific individuals. Overwintering ladybirds were found on tree trunks at different altitudes ranging from 1420-1520 m. Samples were collected from four different locations. The specimens were identified based on their morphological appearance and characteristics of genitalia using valid keys. In this study, a total of six species were identified belonging to five genera, three tribe and three subfamilies of ladybirds. Among them two species were recorded from Yazd province for the first time (marked *). The species identified are as follows: \n1. Hippodamia (Adonia) variegata (Goeze)\n2. Adalia bipunctata (Linnaeus)\n3. Oenopia conglobata contaminata (Menetriés)\n4. Scymnus (Pullus) syriacus (Marsh.)\n5. Brumus gebleri (Weise, 1878)*\n6. Brumus undulatus (Weise, 1878) *