Salvadora persica are traditionally used for the treatment of oral infections. The \nanticandidal; antibacterial and inhibitory effect of S. persica (dry stems) extracts were tested. \nFour fractions were done form miswak, water extract was the highest amount ,then methanol extract , chloroform and petroleum ether gave low amount of extract. The aqueous extract was more efficient than the methanol extract, and chloroform . The strongest antibacterial activity was observed using the aqueous extract against gram positive bacteria strain S. aureus\n(zone of inhibition: 420 mm) and gram ngative bacteria E. coli (zone of inhibition: 360 mm). The aqueous extract had also antifungal activity against C. albicans (zone of inhibition:150mm). The strongest activity was seen against S. pyogenis ,B. subtilis ,S. aureus, L.acidophilus and P. aeruginosa using the water or methanol extract (MIC 1.5 mg /well), followed by S.mutans and E. coli. using the water and methanol extract (MIC 3.5 mg/well).In C. albican the effective MIC at 7mg/well using water and methanol extracts.\nThe inhibitory effect of S. persica extracts on the activities of α-amylase from B.subtilis and α-glucosidase from Saccharomyces cerevisiae. Methanol ,water extracts were showed enzyme inhibitory activity against α -amylase 65and 50 % respectively and inhibition 75-73% against α-glucosidase methanol and aqueous extracts respectively. Thirty three compounds are identified by GC/MS. It was founded that S. persica was rich in benzyl isothiocyanate, benzyl nitrile, benzaldehyde and dimethyl-diheptylphathalate.