Aim: To study the effects of supplementing qi and herbs that promote neovascularization on angiogenesis and expressions of vascular endothelial growth factor (VEGF) and endostatin (ES) protein in experimentally induced acute myocardial infarction (AMI) in rats.\nMethods: Left anterior descending coronary artery was ligated to establish the AMI model of male Sprague-Dawley rats. The rats with AMI were randomly divided into 6 groups，including model group(control), activating blood circulation herbs group (blood group), supplementing qi herbs group(qi group), supplementing qi herbs /activating blood circulation herbs 1:2 group (1:2 Group), supplementing qi herbs/activating blood circulation herbs 2:1 group (2:1 Group) and Tong-xin-luo capsule group(TXL group),and a sham operation group (sham group) set up as negative control group. Microvascular vascular count （MVC） and expressions of VEGF and ES protein were detected and indexes were detected at 7th, 14th and 28th day according to the survival numbers in each group. \nResults: The results showed that supplementing qi in combination with activating blood circulation herbs was better than pure application of supplementing qi and activating blood herbs in promoting angiogenesis through probably increased expression of VEGF. Large doses of qi herbs combined with small doses of activating herbs are superior to small doses of supplementing qi and large doses of activating blood herbs in promoting the angiogenesis, increasing VEGF protein expressions and reducing ES protein expressions. It showed that there was a dose-response effect of supplementing qi and activating blood circulation herbs promoting angiogenesis. Time played an important role in this process. The mechanism of supplementing qi and activating blood circulation herbs and combination of both promoted angiogenesis due to possibly to increased VEGF protein expressions and reduced ES protein expressions. Therefore it seems that qi in combination with herbs that promote blood circulation could be beneficial possibly in patients with acute myocardial infarction (AMI). Further studies may be necessary to validate these findings in experimental animals to human patients.